Abstract Search

Background: The purpose of this study was to register the frequency and cause of method-dependent interference in the Roche thyroid immunoassays observed in our laboratory.Methods: Serum samples to confirm discordant thyroid results were collected from June 2013 to September 2018, in samples sent to the Hormone Laboratory, Oslo University Hospital. The laboratory serves as a reference laboratory on thyroid diseases for about 2/3 of the inhabitants of Nor...

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ea0063p436 | Adrenal and Neuroendocrine Tumours 2 | ECE2019

Measuring steroids hormones by liquid chromatography–tandem mass spectrometry in 21-hydroxylase deficiency (21OHD) reveal large interindividual differences in hormone levels amongst patient with the same genetic mutation

Thorsby Per Medboe , Dahl Sandra R. , Bronstad Ingeborg , Lovas Kristian , Nermoen Ingrid

Background: We have previously shown that LC-MS/MS analyses of steroid hormones are superior to immunoassays monitoring 21OHD, due to better specificity and the possibility to multiplexing several steroid hormones in the same assay. This may lead to better understanding of the individual steroid hormone profile in patients with CAH. Here we present four patients cases with the same genotype in the CYP21A2 gene, and evaluate their steroid profile by LC-MS/MS analyses.<...

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ea0073aep26 | Adrenal and Cardiovascular Endocrinology | ECE2021

Adrenal steroid profiling in the diagnostics of partial enzyme defects in the adrenals. Establishment of normal cut-off levels using LC-MS/MS

Grethe Åstrøm Ueland , Dahl Sandra R. , Methlie Paal , Husebye Eystein , Thorsby Per Medboe

BackgroundNon-classical congenial adrenal hyperplasia (NCCAH) is an important differential diagnosis in women with acne, hirsutism, menstrual abnormalities and infertility. To diagnose NCCAH can be challenging, and currently used cutoff levels are based on unstandardized immunological assays, no longer in use.ObjectiveDefine LC-MS/MS based cut-off levels for steroid hormones, to improve diagnosis of NCCAH and...

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Endocrine Abstracts

Effect of anti-streptavidin interference when diagnosing thyroid disease using immunoassay analyses

Measuring steroids hormones by liquid chromatography–tandem mass spectrometry in 21-hydroxylase deficiency (21OHD) reveal large interindividual differences in hormone levels amongst patient with the same genetic mutation

Adrenal steroid profiling in the diagnostics of partial enzyme defects in the adrenals. Establishment of normal cut-off levels using LC-MS/MS

BiosciAbstracts